Focurose 6FF is designed for component separation and moderate purification of biological macromolecules by removing small molecular impurities. It is suitable for applications such as virus particles, large protein molecules, supercoiled DNA, polysaccharides, and macromolecular complexes.
Features
High (physicochemical) stability, high flow rate (component separation), and high recovery rate (up to 95%)
Gentle elution conditions to preserve the biological activity and functionality of biological macromolecules
Easy to scale up
Easy to maintain
Focurose 6FF Performance Parameters
Resin | Highly cross-linked 6% agarose |
Particle size range | 45-165µm |
Average particle size (D50) | 90±5µm |
Separation range (globular proteins) | 10-4000 kDa |
pH stability | 2-12 (long-term) 2-14 (short-term) |
Chemical stability | 2M sodium hydroxide, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6M guanidine hydrochloride, 8M urea |
Linear flow velocity (0.3 MPa) | ≥300 cm/h |
Maximum pressure | 0.3 MPa |
Storage solution | 20% ethanol |
Storage conditions | 4℃-30℃ |
Frequently Asked Questions and Solutions
Issue | Possible Causes | Solutions |
Rapid increase in chromatographic peak | Overly tight packing of the resin | Repack the column |
Slow or tailing chromatographic peak | Loose packing of the resin | Repack the column |
Cracks or dryness in the column bed | Leakage or introduction of large air bubbles | Check for leaks or bubbles in the tubing and repack the column if necessary |
Poor separation efficiency | 1. Inappropriate choice of matrix | Verify if the selected matrix is suitable |
2. Poor column performance | Evaluate the column efficiency | |
3. Oversampling | Optimize the optimal sampling volume | |
4. Excessive flow rate | Optimize the optimal flow rate | |
Slow liquid flow | 1. Protein or lipid aggregation | Clean the matrix or filter membrane promptly |
2. Protein precipitation in the matrix | Adjust the composition of equilibration and elution buffers to maintain the stability of the target substance and the binding efficiency of the matrix | |
3. Microbial growth in the separation column | Reagents used must be filtered and degassed; samples must be centrifuged or filtered before loading onto the column |
Order information
Product | Specification | Item number |
Focurose 6FF | 25mL | HN060307025M |
Focurose 6FF | 100mL | HN060307100M |
Focurose 6FF | 500mL | HN060307500M |
Focurose 6FF | 1L | HN060307001L |
Focurose 6FF | 5L | HN060307005L |
Focurose 6FF | 20L | HN060307020L |
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