Particle size range  

45-165µm

Average particle size (D50)

90±5µm

Binding capacity

≥10 μmol (epoxy groups) /mL

pH stability

2-14 (long-term) 2-14 (short-term)

Linear flow velocity (0.3 MPa)

≥250 cm/h

Operating pressure

≤0.3MPa

Storage solution

20% ethanol

Storage conditions

4℃-8℃

Coupling efficiency is low

1. Incorrect salt concentration or pH of Buffer B

Check if Buffer B is prepared correctly, ensuring the appropriate salt concentration and pH

2. Insufficient coupling time

Extend the coupling time to allow for a more thorough coupling reaction

3. Inappropriate pre-activated resin

Try using other types of pre-activated resins that may be more suitable for the desired coupling reaction

Target protein does not bind or has low binding capacity during purification

1. Overloading of sample

Reduce the sample load

2. Sample flow rate is too fast

Lower the sample flow rate

3. Protein or lipids aggregate in the resin, affecting binding

Efficiently clean the resin or replace with a new resin

4. Sample deactivation during storage or loading

Properly store the sample to maintain its activity before purification

5. Low binding ratio between ligand and target molecule

Try increasing the ligand concentration during coupling to improve the binding ratio

6. Degradation of ligand during coupling or washing steps

Evaluate the stability of the ligand during the coupling or washing process

Not collecting the target protein during elution or collecting only a small amount of the target protein.

1. Target protein does not bind to the resin or has low binding capacity

First, confirm if the target protein binds to the resin

2. Unsuitable elution conditions

Increase the concentration of imidazole in the elution buffer

3. The target protein aggregates and precipitates under elution conditions

Determine the solubility and stability of the target protein in the elution buffer (pH and salt concentration).

Target protein purity is low

1. Sample not pre-processed

The sample must be centrifuged or filtered before loading onto the column

2. The sample has high viscosity

Dilute the sample with an appropriate equilibration buffer to reduce viscosity

3. Incomplete removal of impurities

Increase the washing volume until the baseline stabilizes and matches the equilibration buffer

4. Impurities such as proteins or lipids aggregate and precipitate in the resin

Clean the resin promptly and effectively

5. Poor elution conditions, such as excessive elution speed or steep gradient

Adjust the elution conditions to improve the elution efficiency

6. Degradation of the target substance

Assess the stability of the target substance

7. Poor column packing

Repack the column or purchase a new one

8. Non-specific adsorption between impurities and the resin

Select appropriate additives to reduce non-specific adsorption

9. Large sample volume stored at the top of the separation column

Repack the column or reduce the sample storage volume

10. Microbial growth in the resin

After using the resin, store it correctly and promptly to prevent microbial growth.

Decrease in resin loading

1. Too fast sample flow rate

Reduce the sample flow rate

2. Aggregation of proteins or lipids in the resin resulting in decreased loading

Clean the resin promptly

3. Excessive use

Replace with a new resin

4. Sample deactivation during storage or loading, inability to bind effectively with the ligand

Properly store the sample to maintain its activity before purification

Rapid increase in chromatographic peak

Overly tight packing of the resin

Repack the column

Slow or tailing chromatographic peak

Loose packing of the resin

Repack the column

Cracks or dryness in the column bed

Leakage or introduction of large air bubbles

Check for leaks or bubbles in the tubing and repack the column if necessary

Slow liquid flow

1. Aggregation of proteins or lipids

Clean the resin or membrane promptly

2. Protein precipitation in the resin

Adjust the composition of the equilibration and elution buffers to maintain the stability of the target substance and the binding efficiency of the resin

3. Microbial growth in the separation column

All reagents used must be filtered and degassed. The sample must be centrifuged or filtered before applying it to the column

Epoxy Focurose 4FF

5mL

HQ030303005M

Epoxy Focurose 4FF

25mL

HQ030303025M

Epoxy Focurose 4FF

100mL

HQ030303100M

Epoxy Focurose 4FF

500mL

HQ030303500M

Epoxy Focurose 4FF

1L

HQ030303001L

Epoxy Focurose 4FF

5L

HQ030303005L

Epoxy Focurose 4FF

20L

HQ030303020L