Serum Marker Kits

HiLA features homogeneous reactions, no washing steps, and perfectly matches the development needs of automated, portable POCT detection instruments. In the IVD field, homogeneous luminescence technology not only ensures the accuracy, reliability, and efficiency of magnetic particle chemiluminescence but also meets the needs of emergency testing. Compared to the commonly used fluorescence immunochromatography in POCT testing, homogeneous luminescence offers higher throughput, lower background, better repeatability, higher sensitivity, and a wider detection range, making it an ideal alternative technology.

HiLA VS Fluorescence Immunoassay

High Precision: In fluorescence immunochromatography, the sample first passes through the conjugate pad and chromatographs through capillary action, then reacts with the antibody fixed on the test line. This non-liquid phase reaction is insufficient and results are poorly reproducible. In HiLA detection, the sample immediately reacts with the detection reagents in a homogeneous system, resulting in a simple and sufficient reaction process with higher precision.

High Sensitivity: HiLA technology uses a nano-beads reaction mode, increasing the reaction surface area. Meanwhile, using 680nm long-wavelength excitation and 615nm short-wavelength emission, combined with time-resolved fluorescence detection mode, effectively reduces background signal and enhances anti-interference capability, coupled with no washing, thereby improving detection sensitivity.

High Throughput: The rapid HiLA reaction and fast detection speed eliminate washing steps, making the instrument free from setting up a liquid system, allowing fully automated operation, and providing higher detection throughput compared to fluorescence immunochromatography.

Serum Marker Kits Ordering Information

Serum Marker Kits Performance

Note: Performance data shown is for a single batch of reagents.

Cytokine Kits

Cytokines are a group of low molecular weight (approximately 6-70kDa) soluble proteins secreted by various cells such as lymphocytes, macrophages, natural killer (NK) cells, mast cells, and stromal cells.^[1] Different cell types can secrete the same cytokine, and a single cytokine can act on various cell types^.[2] Cytokines play a crucial role in immune responses and are important mediators of the immune system's communication network. Cytokines are involved in the pathophysiological processes of viral and bacterial infections, as well as various diseases, including cancer, autoimmune diseases, neurodegenerative diseases, and cytokine storms caused by cell therapies, which have gained significant attention recently. As a result, they have become a focus of medical research.

Cytokine Network^[2]

Product Advantages

l Homogeneous, no-wash reaction model,convenient and efficient, compatible with automated instruments.

l  Wide dynamic range,small sample volume required, suitable for high-throughput rapid testing.

l  High sensitivity and strong anti-interference ability, stable performance and excellent reproducibility.

l  High sample compatibility,applicable to cell culture supernatants, serum, and other natural samples.

Cytokine Kits Ordering Information

Application

VDO has launched several cytokine kits based on HiLA methods. These kits can quantitatively detect cytokine levels in cell culture supernatants or serum and serve as an efficient alternative to traditional methods such as ELISA. They are applicable in various research areas, including basic research, drug immunogenicity evaluation, vaccine development, and more.

Detection Principle

HiLA is a homogeneous immunoassay method based on energy transfer between acceptor and donor beads. This series of kits uses a dual antibody sandwich homogeneous chemiluminescence method to determine cytokine concentrations. Taking the Human IL-6 (hIL-6) kit as an example: The core components of the kit include acceptor beadsconjugated hIL-6 antibody 1 (R1), biotin-labeled hIL-6 antibody 2 (R2), and streptavidin conjugated to the donor beads (R3). In the detection system, acceptor beads-conjugated antibody 1 and biotin-labeled antibody 2 mix and incubate with the analyte, forming a dual antibody sandwich immune complex. This is then reacted with the donor bead to form a luminescent complex. When the distance between the two beads is less than 200 nm, excitation light causes the donor bead to produce singlet oxygen, which diffuses to the acceptor beads. The acceptor beads receives energy and emits light. The light signal is collected by a photodetector, and the concentration of hIL-6 in the sample is calculated by mathematical fitting. If hIL-6 is not present in the sample, no immune complex will form, and the distance between the beads will be greater than 200 nm, exceeding the transfer distance of singlet oxygen, so the acceptor beadsd will not emit light.

Detection Process

In the detection process, 10 μL of sample and pre-mixed R1 and R2 kits are added and incubated at 37°C for 15 minutes. After adding R3 kit in the dark, the mixture is incubated for an additional 10 minutes before detection. Compared to traditional ELISA kits, this detection process eliminates the need for repeated washing of plates, simplifies operations, and significantly reduces labor and time costs.

Performance Demonstration (Partial)

This series of kits offers a wider detection range and can effectively reduce sample dilution steps, saving time.

HiLA Toolbox

HiLA has been widely applied in scientific research. With high sensitivity, precision, and throughput, it is suitable for drug screening, protein-protein interaction, kinase and enzyme activity assays, cell signaling pathway studies, exosome quantification, and more.

In antibody screening applications, HiLA offers the following advantages compared to traditional ELISA technology:

• High sensitivity and wide linear range

• Short detection time and simple process

• No need for coating, requiring fewer types of materials

• Requires less antigen-antibody usage, reducing raw material costs

 HiLA Toolbox Ordering Information

References

[1] Liu C,Chu D,Kalantar-Zadeh K,George J,Young HA,Liu G.Cytokines:From Clinical Significance to Quantification. Adv Sci (Weinh).2021;8(15):e2004433.

[2] Zhang JM,An J.Cytokines,inflammation,and pain.Int Anesthesiol Clin.2007;45(2):27-37.