Particle   size range 

45-165µm

Average   particle size (D50)

90±5µm

Binding   capacity

≥30 mg/mL (for pancreatic protease)

pH   stability

1-9 (short-term), 2-8 (long-term)

Chemical   stability

Compatible with commonly used buffer   solutions, 8M urea, 6M guanidine hydrochloride.

Linear   flow velocity (0.3 MPa)

≥250 cm/h

Operating   pressure

≤0.3MPa

Storage   solution

0.05M NaAc in 20% ethanol pH 4.0

Storage   conditions

4℃-8℃

Target protein does not bind or has low binding capacity during   purification

1.      Overloading of sample

Reduce the sample load

2.      Sample flow rate is too fast

Lower the sample flow rate

3.      Protein or lipids aggregate in the resin, affecting   binding

Efficiently clean the resin or replace with a new resin

4.      The pH of the sample or equilibration buffer is not   within the correct range

Ensure that the pH of the sample and equilibration buffer is within   6.5-8.0

Not collecting the target protein during elution or collecting only a   small amount of the target protein.

1.      Target protein does not bind to the resin or has low binding capacity

First, confirm if the target protein binds to the resin

2.      Unsuitable elution conditions

The elution buffer needs to be replaced

3.      Insufficient elution time

Lower the flow rate and extend the retention time of the elution   buffer

4.      Elution volume is too small

Increase the elution volume

5.      The target protein aggregates and precipitates under   elution conditions

Assess the solubility and stability of the target analyte under the   elution buffer conditions (pH)

Target protein purity is low

1.      Sample not pre-processed

The sample must be centrifuged or filtered before loading onto the   column

2.      The sample has high viscosity

Dilute the sample with an appropriate equilibration buffer to reduce   viscosity

3.      Incomplete removal of impurities

Increase the washing volume until the baseline stabilizes and matches   the equilibration buffer

4.      Impurities such as proteins or lipids aggregate and   precipitate in the resin

Clean the resin promptly and effectively

5.      The elution conditions are not optimal, with a too   fast elution flow rate and steep gradient

Optimize the elution conditions

6.      Degradation of the target substance

Assess the stability of the target analyte

7.      Poor column packing

Repack the column or purchase a new one

8.      Non-specific adsorption between impurities and the resin

Appropriately select additives to reduce non-specific adsorption

9.      Large sample volume stored at the top of the   separation column

Repack the column or reduce the sample storage volume

10.   Microbial growth in the resin

After using the resin, store it correctly and promptly to prevent microbial   growth.

Decrease in resin loading

1.      Too fast sample flow rate

Reduce the sample flow rate

2.      Aggregation of proteins or lipids in the resin resulting in decreased loading

Clean the resin promptly

3.      Excessive usage may result in oxidation or detachment   of the ligand

It is recommended to clean the medium promptly or replace it with a   new one

Rapid increase in chromatographic peak

Overly tight packing of the resin

Repack the column

Slow or tailing chromatographic peak

Loose packing of the resin

Repack the column

Cracks or dryness in the column bed

Leakage or introduction of large air bubbles

Check for leaks or bubbles in the tubing and repack the column if   necessary

Slow liquid flow

1.      Aggregation of proteins or lipids

Clean the resin or membrane promptly

2.      Protein precipitation in the resin

Adjust the composition of the equilibration and elution buffers to   maintain the stability of the target substance and the binding efficiency of   the resin

3.      Microbial growth in the separation column

All reagents used must be filtered and degassed. The sample must be   centrifuged or filtered before applying it to the column

Benzamidine Focurose 4FF(HS)

25mL

HQ030317025M

Benzamidine Focurose 4FF(HS)

100mL

HQ030317100M

Benzamidine Focurose 4FF(HS)

500mL

HQ030317500M

Benzamidine Focurose 4FF(HS)

1L

HQ030317001L

Benzamidine Focurose 4FF(HS)

5L

HQ030317005L

Benzamidine Focurose 4FF(HS)

20L

HQ030317020L