VDO biotech’s magnetic microsphere for viral nucleic acid is a core-shell structure and polydisperse magnetic microsphere, and the surface is coated with silicon hydroxyl groups. MS02HA has fast magnetic response speed, reliable suspension, and strong performance hydrophilicity and nucleic acid capture ability. MS02HA is highly efficient in extracting nucleic acids from virus, pseudovirus particles and small fragments. It is suitable for a variety of sample types, suitable for automated nucleic acid extraction, and is an ideal choice for biological sample purification.
Product Features
● Large specific surface area: the high capability to combine with nucleic acid
● High hydrophilic property: reduce the possibility of the beads adhering to the tube walls
● Multiple sample compatibility: are suitable for the virus, pseudoviral and small nucleic acid fragment
● Special chemical groups: the high capacity to adsorb nucleic acid and ease to elute
Technical Parameters
● Particle Size: ~150nm
● Material: SiO2/Fe3O4
● Surface: OH
● Disperse Medium: DI water
● Additives: trace amount of surfactant, 0.1% Proclin 300
Nucleic Acid Extraction Process
The nucleic acid binding to magnetic microspheres relie on electrostatic, hydrogen bond, hydrophobic effects. Utilizing the difference in the physical and chemical properties of nucleic acid and protein, after washing to remove impurities, the target nucleic acid is eluted under a low-salt solution. Nucleic acid extraction by magnetic microspheres consists of four steps: lysis, adsorption, washing, elution. As shown in the figure:
Sample Types and Recommended Magnetic Microspheres
To verify the performance of MS02HA, we utilized the pure nucleic acids and nuclease-free water to simulate virus samples for testing.
● Method: add 200μl (1000 copies/ml) of nucleic acid fragments into a tube, use MS02HA for extraction, the eluate is 100μl of nuclease-free water. And then the pure nucleic acid and the eluate after extraction are amplified under the same conditions.
● Result: the qPCR results showed that the CT value of the pure nucleic acid solution was 0.8 higher than that of eluate, so it has been proved that MS02HA magnetic microspheres have high capture ability for small nucleic acid fragments.
| Extraction Reagent | Magnetic Beads | Quality | Amplification Reagent | Samples | CT Value |
| Reagent A | MS02HA | 20μl | Reagent B | Pure Nucleic Acids | 32.3 |
| Reagent A | MS02HA | 20μl | Reagent B | Pure Nucleic Acids | 31.5 |
Magnetic Microspheres for Nucleic Acid Extraction
| Cat. No. | Color | Surface Groups | Solids | Selected Applications |
| MS02H | Brownish black | OH | 2.5% | Viral nucleic acid extraction |
| MA200H | Brownish black | OH | 2.5% | cfDNA extraction PCR products purificatio |
| MA0308C | Brownish yellow | COOH | 2.5% | Viral nucleic acid extraction |
MA0309C | Brownish yellow | COOH | 2.5% | Viral nucleic acid extraction |
| MS05HC | Brownish yellow | OH | 2.5% | Viral nucleic acid extraction |
MS05HE | Brownish yellow | OH | 3.0% | Viral nucleic acid extraction |
MS04T | Brownish yellow | Oligo(dT) | 1.0% | mRNA extraction from animal and plant samples |
MS02HA | Brownish black | OH | 2.5% | Virus,pseudovirus particles,small fragment nucleic acid extraction |
MS04HC | Brownish black | OH | 2.5% | Viral nucleic acid extraction,throat and nose swab serum,vaginal swabs |
The magnetic microsphere products above are all available in 10ml,100ml,and 1L.
Supporting Raw Material
| Cat. No. | Product Name | Form | CAS | Use | Size |
VYJ1300905 | Guanidine Hydrochloride | White crystal | 50-01-1 | Strong protein allosteric agent | 500g |
VYJ1300910 | 1Kg | ||||
VYJ1301210 | Guanidine Isothiocyanate | White crystal | 593-84-0 | Strong protein allosteric agent | 1Kg |
VYJ13012250 | 25Kg | ||||
| PK0030 | Proteinase K | White lyophilized powder | 39450-01-6 | Cell lysis | 30mg |
| PK0100 | 100mg | ||||
| PK1000 | 1000mg | ||||
| PK1050 | 50g |
Supporting Consumables
| Product Name | Size |
96-Well Deep Well Plates | A variety of packaging specifications are available. |
96-Well Plates | |
96-Well Magnetic Rod Cover | |
8-Well Magnetic Rod Cover | |
96 Sealing Film |
If you have questions related to product information, or would like to request samples, quotations or demo, please submit your inquiry.
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