Particle size range  

25-45 µm

Average particle size (D50)

35±5 µm

Separation Range (Globular Proteins)

3-70kDa

pH stability

3-12 (long-term) 1-14 (short-term)

Chemical stability

Compatible with all common solutions including 8M urea, 6M guanidine hydrochloride, 70% ethanol, and 1M sodium hydroxide

Storage solution

0.2M NaAC, 20% ethanol

Storage conditions

4℃-30℃

Resolution difference between target peak and impurity peak

1. Overloading of sample

Reduce the sample volume to 0.5% of the column volume (CV)

2. Sample too viscous

Dilute the sample appropriately

3. Excessive flow rate

Reduce the flow rate

4. Short column length 

Choose a longer or narrower column

5. Excessive dead volume

Minimize dead volume in tubing and connectors

6. Poor column packing

Repack the column or use prepacked columns

7. Unfiltered sample

Filter the sample using a 0.22 µm or 0.45 µm filter

8. Contaminated resin

Clean the column and re-equilibrate

9. Improper column installation

Reinstall the column vertically

10. Non-uniform temperature usage

Maintain a constant temperature throughout the process

Unexpected elution peak

1. Inconsistent sample volume

Maintain the same sample volume throughout

2. Ionic interaction between protein and resin

Maintain the ionic strength of the buffer in the range of 0.05-0.15 NaCl

3. Ionic interaction between protein and resin

Reducing the ionic strength can minimize hydrophobic interactions. Additionally, hydrophobic interactions can be reduced by increasing the pH, adding surfactants, or incorporating organic reagents

4. Changes in the sample during storage

Prepare fresh samples

5. Precipitation of proteins and lipids in the chromatography column

Clean the column or replace it with a new one

6. Microbial growth in the resin

The column does not support microbial growth during usage, but it should be stored in 20% ethanol

Washing peak elution occurs earlier

1. Gaps in the packed column

Repack the column

2. Formation of protein dimers or oligomers

Ensure the stability of the sample under experimental conditions

Delayed elution of the washing peak

1. Ionic or hydrophobic interactions between proteins and the resin

Maintain an ionic strength of 0.05-0.15M NaCl in the buffer

2. Dirt on the resin, filter membrane, or top of the column bed

Clean the column and re-equilibrate

3. Microbial growth in the resin

The column does not support microbial growth during usage, but it should be stored in 20% ethanol

Rapid increase in chromatographic peak

Overly tight packing of the resin

Repack the column

Slow or tailing chromatographic peak

Loose packing of the resin

Repack the column

Cracks or dryness in the column bed

Leakage or introduction of large air bubbles

Check for leaks or bubbles in the tubing and repack the column if necessary

Slow liquid flow

1. Aggregation of proteins or lipids

Clean the resin or membrane promptly

2. Protein precipitation in the resin

Adjust the composition of the equilibration and elution buffers to maintain the stability of the target substance and the binding efficiency of the resin

3. Microbial growth in the separation column

All reagents used must be filtered and degassed. The sample must be centrifuged or filtered before applying it to the column

4. Compression of the column bed

Repack the column

Column bed contains bubbles

Repack the column due to temperature differences during use or residue in the tubing

Repack the column

Increased pressure

1. Sample turbidity

Prepare a fresh sample

2. Clogging in the tubing or frit

Clean the tubing, frit, and resin; repack the column

Focurose 75PG

25mL

HN120209025M

Focurose 75PG

100mL

HN120209100M

Focurose 75PG

500mL

HN120209500M

Focurose 75PG

1L

HN120209001L

Focurose 75PG

5L

HN120209005L

Focurose 75PG

20L

HN120209020L