The Protein A Magnetic Agarose Microspheres productis composed of agarose and nano magnetic particles,with Protein Aligands conjugated to the surface of the beads, When the Protein A Magnetic Agarose Microspheres are added to a sample containing antibodies and incubated,the antibodies bind fully to the agarose magnetic beads, The antibodies bound to the agarose magnetic beads can then be separated magnetically and eluted under acidic conditions. This magnetic separation method eliminates the need for centrifugation steps, reduces purification time, and increases operational convenience, making it more suitable for high-throughput sample purification.

Product Features

High Binding Capacity: Each milliliter of Protein A sediment gel can bind approximately 90 mg of human IgG.

High Purity: For real samples (CHO and HEK293 samples), the eluted purity is ≥90%.

High Alkali Resistance: The ligands have been sequence-optimized to withstand standard CIP procedures with 0.1M NaOH, with a binding capacity reduction of less than 20% after 40 cycles.

Better Suspension: The average particle size is 30 um, providing better suspension and more effective capture of free antibodies.

Better Hydrophilicity: Utilizes self-produced high-hydrophilicity nano magnetic particles, effectively reducing the nonspecific adsorption of the magnetic particles themselves.

Faster Magnetic Response: Can be quickly adsorbed in the solution, with a magnetic response time of ≤ 3 seconds.

Fully Automated Process: Compatible with VDO high-throughput automated purification instruments, enabling full process automation from bead pre-treatment, binding, elution, to bead recovery.

Technical Parameters

• Matrix: Agarose magnetic beads

• Particle size range: 10-50μm

• Average Particle Size: ≈30μm

• Binding Capacity: ≈90mg (human IgG)/mL sediment gel

• pH Stability: 3-9(long-term); 2-10(short-term)

• Chemical Stability: Stable in all common buffer solutions; stable in 70% ethanol and 6M guanidine hydrochloride (for 8 days)

• Microsphere concentration: 25% suspension

• Storage solution: 20% ethanol or 2% benzyl alcohol

• Storage temperature: 2-8℃

Structure Of Magnetic Agarose Microspheres And Antibody Purification Principle

1. Standard Human IgG Testing

Using human IgG standards for load testing, VDO Protein A Magnetic Agarose Microspheres have a binding capacity of 86.07 mg/mL of sediment gel, with a two-step elution load totaling 78.42 mg/mL of sediment gel and a total recovery rate of 91.1%.

2. Alkali Resistance Testing

Using 0.1M NaOH (15 min/cycle) to test the alkali resistance of VDO Protein A Magnetic Agarose Microspheres. After 40 cycles, both the binding capacity and elution load decreased by less than 20%.

3. Protein A Ligand Leaching Test

Protein A Magnetic Agarose Microspheres were washed with 0.1 M NaOH (15 min per cycle).

After 40 cycles, the residual Protein A ligand detected in the eluate was ≤15 ng/mL, indicating excellent ligand stability.

4. Purification of Actual Samples (CHO Cells & HEK293 Cells)

Using VDO Protein A Magnetic Agarose Beads to purify IgG antibodies from CHO and HEK293 cell culture supernatants. Eluted antibodies were separated by SDS-PAGE. VDO Protein A Magnetic Agarose Beads were used to purify CHO cell antibody samples. HPLC analysis showed that the purity of antibody was 26.46% before purification and increased to 92.73% after purification.

VDO Protein A Magnetic Agarose Beads were used to purify HEK293 cell antibody samples. HPLC analysis showed that the purity of antibody was 4.27% before purification, and increased to 94.41% after purification.

5. Comparison with Competitors

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