Product Introduction

The Protein A Magnetic Agarose Microspheres product is composed of agarose and nano magnetic particles, with Protein A ligands conjugated to the surface of the beads. When the Protein A Magnetic Agarose Microspheres are added to a sample containing antibodies and incubated, the antibodies bind fully to the agarose magnetic beads.  The antibodies bound to the agarose magnetic beads can then be separated magnetically and eluted under acidic conditions. This magnetic separation method eliminates the need for centrifugation steps, reduces purification time, and increases operational convenience, making it more suitable for high-throughput sample purification.

Product Features

• High Binding Capacity: Each milliliter of Protein A sediment gel can bind approximately 90 mg of human IgG. 

• High Purity: For real samples (CHO and HEK293 samples), the eluted purity is ≥ 90%. 

• High Alkali Resistance: The ligands have been sequence-optimized to withstand standard CIP procedures with   0.1M NaOH, with a binding capacity reduction of less than 20% after 40 cycles. 

• Better Suspension: The average particle size is 30 µm, providing better suspension and more effective capture  of free antibodies. 

• Better Hydrophilicity: Utilizes self-produced high-hydrophilicity nano magnetic particles, effectively reducing   the nonspecific adsorption of the magnetic particles themselves. 

• Faster Magnetic Response:  

• Fully Automated Process: Can be quickly adsorbed in the solution, with a magnetic response time of ≤ 3 seconds.  Compatible with VDO high-throughput automated purification instruments, enabling   full process automation from bead pre-treatment, binding, elution, to bead recovery.

Product Information