Particle size range  

45-165µm

Average particle size (D50)

90±5µm

Binding capacity

≥20 mg (GST)/mL

Chemical stability

All commonly used aqueous solutions, such as: 1M acetate buffer, pH 4.0, 0.1M NaOH, 70% ethanol, 8M urea, 6M guanidine hydrochloride.

Linear flow velocity (0.3 MPa)

≥250 cm/h

Operating pressure

≤0.3MPa

Storage solution

20% ethanol

Storage conditions

4℃-30℃

Target protein does not bind or has low binding capacity during purification

1. Overloading of sample

Reduce the sample load

2. Sample flow rate is too fast

Lower the sample flow rate

3. Protein or lipids aggregate in the resin, affecting binding

Efficiently clean the resin or replace with a new resin

4. Sample inactivates during ultrasonic disruption

Use milder conditions for ultrasonic disruption

5. pH of the sample or equilibration buffer is outside the correct range

Ensure that the pH of the sample and equilibration buffer is within 6.5-8.0

6. Expression conditions are too harsh, causing conformational changes in the target molecule, preventing its binding to the matrix

It is recommended to include an empty vector as a control for expression and purification

7. Target substance aggregates

Add 1-10 mM DTT before disruption

Not collecting the target protein during elution or collecting only a small amount of the target protein.

1. Target protein does not bind to the resin or has low binding capacity

First, confirm if the target protein binds to the resin

2. Unsuitable elution conditions

Increase the concentration of GSH to 20-40 mM and ensure that the elution buffer has a pH within the range of 8.0-9.0

3. Insufficient elution time

Lower the flow rate and extend the retention time of the elution buffer

4. Elution volume is too small

Increase the elution volume

5. The target protein aggregates and precipitates under elution conditions

Determine the solubility and stability of the target protein in the elution buffer (pH and salt concentration). Try adding some additives to the elution buffer, such as 0.1%Triton X-100 or 2%N-octyl glucoside

Target protein purity is low

1. Sample not pre-processed

The sample must be centrifuged or filtered before loading onto the column

2. The sample has high viscosity

Dilute the sample with an appropriate equilibration buffer to reduce viscosity

3. Incomplete removal of impurities

Increase the washing volume until the baseline stabilizes and matches the equilibration buffer

4. Impurities such as proteins or lipids aggregate and precipitate in the resin

Clean the resin promptly and effectively

5. Poor elution conditions, such as excessively fast flow rate or steep gradient

Optimize the elution conditions

6. Degradation of the target substance

Assess the stability of the target substance

7. Poor column packing

Repack the column or purchase a new one

8. Non-specific adsorption between impurities and the resin

Select appropriate additives to reduce non-specific adsorption

9. Large sample volume stored at the top of the separation column

Repack the column or reduce the sample storage volume

10. Microbial growth in the resin

After using the resin, store it correctly and promptly to prevent microbial growth.

Decrease in resin loading

1. Too fast sample flow rate

Reduce the sample flow rate

2. Aggregation of proteins or lipids in the resin resulting in decreased loading

Clean the resin promptly

3. Excessive usage causing oxidation or detachment of the ligand

Clean the matrix promptly or replace with a new matrix

Rapid increase in chromatographic peak

Overly tight packing of the resin

Repack the column

Slow or tailing chromatographic peak

Loose packing of the resin

Repack the column

Cracks or dryness in the column bed

Leakage or introduction of large air bubbles

Check for leaks or bubbles in the tubing and repack the column if necessary

Slow liquid flow

1. Aggregation of proteins or lipids

Clean the resin or membrane promptly

2. Protein precipitation in the resin

Adjust the composition of the equilibration and elution buffers to maintain the stability of the target substance and the binding efficiency of the resin

3. Microbial growth in the separation column

All reagents used must be filtered and degassed. The sample must be centrifuged or filtered before applying it to the column

GST Focurose 4FF

1mL

HQ030307001E

GST Focurose 4FF

5mL

HQ030307005E