VDX MMA ultra——High Salt Tolerance Mixed Mode Series
VDX MMA ultra is a high-resolution multimodal chromatography resin. This chromatography resin significantly enhances the resolution of separation and purification through special surface modification and ligand structure optimization, making it particularly suitable for the polishing of biomolecules under high salt conditions (removal of antibody dimers, aggregates, host cell proteins and nucleic acids, etc.).
What's the difference between VDX MMA ultra and traditional ion exchange resin?
MMA ligand enables various interactions with target molecules, primarily via strong anion exchange, alongside hydrogen bonding and hydrophobic interactions. VDX MMA ultra, a high salt-tolerant multi-mode medium in the VDX series, offers unique selectivity. Unlike traditional ion exchange media, it efficiently binds target proteins under high-conductivity conditions, enhancing purification efficiency for complex samples. This makes it ideal for purifying monoclonal antibodies, recombinant proteins, and other biopharmaceuticals.
High Salt Tolerance: Enables efficient target binding at high salt concentrations, minimizing sample dilution requirements.
High Recovery Rates: Outperforms comparable products with its reliable protein recovery efficiency.
Unique Selectivity: Combines hydrophobic and ion-exchange interactions for enhanced specificity in protein adsorption.
| VDX MMA ultra Performance Parameters | |
Matrix | High rigidity agarose |
Particle size range | 45-165µm |
Particle size, d50v | 70±10µm |
Ion exchange type | Multimodal strong anion exchanger |
Ion capacity | 80-120μmol Cl-/mL resin |
Dynamic binding capacity* | 50mg BSA/mL resin |
pH stability | 4-13(long term) /2-14(short term) |
Operating pressure | ≤0.3MPa |
Recommended flow velocity | 200-300cm/h |
Chemical stability | Stable to commonly used aqueous buffers, 1.0 M NaOH, 8 M Urea, 6 M guanidine hydrochloride, 70% ethanol; Avoid oxidants, anionic detergents |
Storage conditions | 20% ethanol or 2% benzyl alcohol |
Storage temperature | 4-30°C |
*DBC: 10% breakthrough capacity determined using 5mg/mL of BSA in 20mM Bis-Tris, pH6.5 with 2 min residence time.
Application Cases——Separation of Antibody Monomers and Aggregates Using VDX MMA ultra
Buffer A: 20 mM PB, pH 7.0
Buffer B: 20 mM PB, 1 M NaCl, pH 7.0
Elution: 60% Buffer B for 3 CV
Regeneration: 1 M Acetic acid for 3 CV
Result: Purification using VDX MMA Ultra achieved a purity of 98.96% and a recovery rate of 72%.
| VDX MMA ultra Order Information | |||
Product Name | Specification | Product Number | Application Characteristics |
VDX MMA ultra | 25 mL | HF190826025M | High-resolution for antibody aggregates separation |
100 mL | HF190826100M | ||
500 mL | HF190826500M | ||
1 L | HF190826001L | ||
5 L | HF190826005L | ||
20 L | HF190826020L | ||
| Pre-assembled Column Ordering Information | ||
| Product Name | Specification | Item Number |
| VDX MMA ultra | 1mL | HF190826001E |
| VDX MMA ultra | 5mL | HF190826005E |
If you have questions related to product information, or would like to request samples, quotations or demo, please submit your inquiry.
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