Suzhou Vdo Biotech Co.Ltd.

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Flow cytometry application

Flow cytometry application:

Flow cytometry is an ideal technology for the counting, identification and classification of particles suspended in sheath liquid. By adding the absolute count microspheres equal to the volume of test sample and mixing thoroughly before detecting by device, according to the number of known absolute count the number of microspheres, the number of sample cells in a certain volume can be calculated; and by combining flow cytometry antibody clustering method, the absolute number of a group cells in a certain volume can be calculated.

Technical parameters:
Material: multiple fluorescence-stained polystyrene particles
Color: green fluorescence (488/520 nm), red fluorescence (535/610 nm), near-infrared fluorescence (635/700 nm)
Concentration: 50000 particles/mL ± 5%
Density: 1.05g/cm3
Additives: 0.05% tween-20 dispersant/surfactant
Storage conditions: 2 - 8°C, be sure not to freeze  

Product application

Instrument quality control of flow cytometry includes verifications of sensitivity, fluorescence, fluorescence detector and photomultiplier performance. The instruments should be regularly monitored using flow cytometry microspheres, so that the instrument continuity can be guaranteed over time, and the performance change of instruments should be monitored too, to ensure the reliability of the data. The fluorescence microspheres we provide that wrap stable fluorescent dye inside create an ideal material for long-term performance detection of single instrument in different laboratory. Those microspheres will test and define the optical and sheath flow conditions, thus ensuring the good performance of instruments to analyze different cell populations.

Applicable models

This series of products are suitable for BD Biosciences’ BD FACSCalibur™ and BD FACSAria IIand Beckman Coulter's Cytomics FC 500.

 

Diameter C.V. Packing Size Product description Catlog
3μm Within 3% 50000 pcs/tube Absolute counting quality control FC03
4μm Within 3% 50000 pcs/tube Absolute counting quality control FC04
5μm Within 3% 50000 pcs/tube Absolute counting quality control FC05
6μm Within 3% 50000 pcs/tube Absolute counting quality control FC06
7μm Within 3% 50000 pcs/tube Absolute counting quality control FC07

 

Other specifications can be tailored according to customer requirements

Suzhou Vdo Biotech Co.Ltd.